This statement describe the incident that happens in my lab today..though its a no big deal case, but i am in such a despair and gloominess now..=((
This is what happens;
on a blue Monday,
the time slot i want to book in da lab on tuesday has been booked!
then how shall i do my MTT assay?
then how shall i do my MTT assay?
FYI: each MTT assay took three days to complete..day 1: seeding the cells, then incubate for 18 hours..day 2: treatment with desired compounds, then incubate for 24 hours..day 3: MTT salt addition
i AM SO CONFIDENT that this time it will be the most good looking result.based on the experience on the doing the previous MTTs, .i tried my very best to arrange the exactly 18 hours of incubation time for my beloved seeded cells, i am so confident that i am gonna get the timing damn accurate(perfect!) this time, EXACTLY 18 hours!, then on Monday, my desired time has been booked, nevermind.
i AM SO CONFIDENT that this time it will be the most good looking result.based on the experience on the doing the previous MTTs, .i tried my very best to arrange the exactly 18 hours of incubation time for my beloved seeded cells, i am so confident that i am gonna get the timing damn accurate(perfect!) this time, EXACTLY 18 hours!, then on Monday, my desired time has been booked, nevermind.
On that time in the lab, i carried on my seeding,(my mind running on a treadmill, and my thoughts were planning non stop) and luck seems to be by my side at that time, i seeded later than the time i expected, so i manage to exchange time slots on the next day with 2 lab mates, and i've got my desired time!! to be able to treat after incubating exactly 18 hours!! Day 2, all's well ends well! I am very proud of myself for being able to arrange and negotiate well to get my desired time slot!! I am very thankfull to the 2 lab mates as well<3
then Day 3, which is today, everything goes very well too, the micropipettes seems to behave very well, and i sort of gain the golden key to using those pipetes in the most efficient way..I am very happy indeed, as i have mastered the skill (hehe) to avoid any medium remaining on the pipettes..ngek..>)....then my cells and the treatment compOund behave as well, the colours of the solution in each well is nice, and i have so much confident that its gonna come out BEAUTIFULLY...
then, after performing the last step which is to suspend the formazans with DMSO, its time to read the plate..everything is according to plan, and i feel as if i m walking on rainbow, plus this is my last MTT assay i need to do at the time being, i cant wait to say goodbye to it, plus its gonna end WELL!!!..
then just when i was about to read the plate using the microtiter plate reader(imma walking towards the reader thinking"this is gonna be a good one, hell yeah"), my inner voice asked to shake the plate using the shaker to mix well the solutions, and in order for more perfectness, i decided to use the shaker before i read it..(just for the purpose of perfectness)..
and then...my nightmare, happensT.T the shaker aint shaking , and just when i turned the mode to make it shake, it starts to shake vigorously!T.T and in the end, my babies!! all my babies in the well flew and spill everywhere like nobody's business...T.T (gosh, i am in the verge of tears now)..IN-THE-END, everything is ruined, it cant be read anymore as everything is mixed up and spilled..omg, my heart hurts like as if someone has cut a piece of myocardium away..
Hence, i threw everything away to the biohazard bag, kissing them goodbye, and wipe the spills away from the effin shaker with mixed feelings of defeat and failure;..i suddenly feel blank and numb, standing in a corner and thinking how great my day is gonna end up, if i didnt use the shaker, and i could have just read the plate, get the beautifully predicted result, and hence end my MTT pipeting and suspending torment, and also fills up the last perfect puzzle to my MTT data analysis..but everything comes to a halt in just less than five seconds or so i think..=(((
i stood there flabbergasted and kept thinking of the IFs, until Dr C saw me and said to me, "U feeling depressed because of what happened just now? " then , me replying"A bit"..actually its just not a bit , i just feel like banging myself to the wall or all drink up all the DMEM solutions that time, but i cant because i still love my head, and i couldnt afford to waste the precious DMEM solutions..
Hence, even though i am still brooding over my stillbirth results, well, i guess i have to repeat it again next week..i try to think it in every possible positive ways..such as, maybe God want it to happen so that i am able to produce a BETTER result next week, perhaps the best and better than what could be today's ! and since my cell viability on that time was 86% and Miss E says that its better if its at least 90%, this is another reason why God wants me to repeat the assay next week!! =)) and please, God bless me please, next week , please bless me so that the compounds RV and LPS are still enough for me to use, as for RV, i am gonna do 2n using Dr C's cell and 1n using my cells, while for LPS(hope that its enuf), i am gonna do 1n each using Dr C and my cells..YEAH!! maybe all this happens,because fate wants me to repeat with more n-sss so that i will produce better groups of result, and fuck yeah! i am so gonna take this opportunity, and being able plus having a reason to repeat MTT assay again to improve my Datasss!!
I will not give up and this time, i will make it better!! BETTER EACH TIME~
peace!
then Day 3, which is today, everything goes very well too, the micropipettes seems to behave very well, and i sort of gain the golden key to using those pipetes in the most efficient way..I am very happy indeed, as i have mastered the skill (hehe) to avoid any medium remaining on the pipettes..ngek..>)....then my cells and the treatment compOund behave as well, the colours of the solution in each well is nice, and i have so much confident that its gonna come out BEAUTIFULLY...
then, after performing the last step which is to suspend the formazans with DMSO, its time to read the plate..everything is according to plan, and i feel as if i m walking on rainbow, plus this is my last MTT assay i need to do at the time being, i cant wait to say goodbye to it, plus its gonna end WELL!!!..
then just when i was about to read the plate using the microtiter plate reader(imma walking towards the reader thinking"this is gonna be a good one, hell yeah"), my inner voice asked to shake the plate using the shaker to mix well the solutions, and in order for more perfectness, i decided to use the shaker before i read it..(just for the purpose of perfectness)..
and then...my nightmare, happensT.T the shaker aint shaking , and just when i turned the mode to make it shake, it starts to shake vigorously!T.T and in the end, my babies!! all my babies in the well flew and spill everywhere like nobody's business...T.T (gosh, i am in the verge of tears now)..IN-THE-END, everything is ruined, it cant be read anymore as everything is mixed up and spilled..omg, my heart hurts like as if someone has cut a piece of myocardium away..
Hence, i threw everything away to the biohazard bag, kissing them goodbye, and wipe the spills away from the effin shaker with mixed feelings of defeat and failure;..i suddenly feel blank and numb, standing in a corner and thinking how great my day is gonna end up, if i didnt use the shaker, and i could have just read the plate, get the beautifully predicted result, and hence end my MTT pipeting and suspending torment, and also fills up the last perfect puzzle to my MTT data analysis..but everything comes to a halt in just less than five seconds or so i think..=(((
i stood there flabbergasted and kept thinking of the IFs, until Dr C saw me and said to me, "U feeling depressed because of what happened just now? " then , me replying"A bit"..actually its just not a bit , i just feel like banging myself to the wall or all drink up all the DMEM solutions that time, but i cant because i still love my head, and i couldnt afford to waste the precious DMEM solutions..
Hence, even though i am still brooding over my stillbirth results, well, i guess i have to repeat it again next week..i try to think it in every possible positive ways..such as, maybe God want it to happen so that i am able to produce a BETTER result next week, perhaps the best and better than what could be today's ! and since my cell viability on that time was 86% and Miss E says that its better if its at least 90%, this is another reason why God wants me to repeat the assay next week!! =)) and please, God bless me please, next week , please bless me so that the compounds RV and LPS are still enough for me to use, as for RV, i am gonna do 2n using Dr C's cell and 1n using my cells, while for LPS(hope that its enuf), i am gonna do 1n each using Dr C and my cells..YEAH!! maybe all this happens,because fate wants me to repeat with more n-sss so that i will produce better groups of result, and fuck yeah! i am so gonna take this opportunity, and being able plus having a reason to repeat MTT assay again to improve my Datasss!!
I will not give up and this time, i will make it better!! BETTER EACH TIME~
peace!
and oh well, i have thought of a name for my stilbirth result for today, though to u it might not be a big deal, but to me, after putting so much effort arranging the time, negotiating to get my desired time(u have no idea how many litres of brain juice i have squeezed to plan to get my desired time slot) and taming the micropipettes to behave well, and this is all in order to gain PERFECTNESS..i am so close to it, u know? SOOOO CLOSEEEEE.. everything, all the steps been fulfiiled perfectly, only to fail and collapse at reading the plate! READING-THE PLATE-CAN U IMAGINE IT? JUST PUTTING IT IN THE READER, AND USE COMPUTER TO READ IT, IT DOESNT REQUIRE ANY SPECIAL TECHNIQUES, not like pipetting, or preparing the calculated volumes of solution u using and yet it collapsed at the easiest final part, (llife is so HA-HA and i feel so F-ed up that time HA.HA.=.=~~~..its just as if u r so close to getting up on the train, but the train left just when u are just a few steps away..sighh~~
but nevermind, even if u missed the train, there is always a next train, perhaps a BETTER and more COMFORTABLE one,..as EVERYTHING TRULY HAPPENS FOR A REASON, FOR GOOD=)..
good, i shalt never give up, and i shall get 90% and above viability next time, continue taming the micropipettes as i have already mastered the skills (ngekk), and continue to strive for the best as usual..!
Peace>.^..
oh i name it, The One That Got Away~~~
but nevermind, even if u missed the train, there is always a next train, perhaps a BETTER and more COMFORTABLE one,..as EVERYTHING TRULY HAPPENS FOR A REASON, FOR GOOD=)..
good, i shalt never give up, and i shall get 90% and above viability next time, continue taming the micropipettes as i have already mastered the skills (ngekk), and continue to strive for the best as usual..!
Peace>.^..
oh i name it, The One That Got Away~~~
lol
~
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